Peptide Reconstitution Calculator
Calculate peptide concentration after reconstitution. Enter vial size and water volume to find mcg per unit on a standard insulin syringe. Free tool.
Reconstitution calculations help in knowing the details of contents in each syringe. Specify the size of the vial, amount of the solvent used and the required amount of dose for determination of concentrations and volumes needed swiftly.
How the Peptide Calculator Works
Understanding the Math Behind Reconstitution
Concentration Formula
Concentration is calculated by dividing the total amount of peptide (in mg) by the volume of water used (in ml). If a vial containing 10 mg was reconstituted with 2 ml of bacteriostatic water, the concentration that will be obtained is 5 mg/ml, and if a vial containing 5 mg was reconstituted with 1 ml, the concentration will still be the same. The factors of peptide weight and volume of water added are completely in your hands, as you will use either more or less water accordingly.
Units on an Insulin Syringe
A typical U-100 insulin syringe is marked so that 100 units equals to 1 mL of liquid. The "unit" markings on the syringe shows the volumes in 0.01 mL of liquid .The amount of peptides in each unit depends solely on the peptide concentration made during reconstitution. At 5 mg/mL concentration, each unit (0.01 mL) would then be equal to 0.05 mg (50 mcg) of peptide. At concentrations of 10 mg/mL, each unit would equal 0.10 mg (100 mcg) of peptide. So it becomes clear that the concentration must be calculated before any measurements can be made.
| Vial Size | Water Added | Concentration | mcg per Unit | Units for 250 mcg |
|---|---|---|---|---|
| 5 mg | 1 mL | 5 mg/mL | 50 mcg | 5 units |
| 5 mg | 2 mL | 2.5 mg/mL | 25 mcg | 10 units |
| 10 mg | 1 mL | 10 mg/mL | 100 mcg | 2.5 units |
| 10 mg | 2 mL | 5 mg/mL | 50 mcg | 5 units |
Bacteriostatic Water for Reconstitution
Bacteriostatic water (BAC water) is a sterile water that contains 0.9% of the preservative benzyl alcohol. The benzyl alcohol prevents the growth of microorganisms, making BAC water ideal for multi-dose vials. You can use BAC water repeatedly for the 28-day period. However, sterile water for injection (without the preservative) is for single use only and is so not appropriate for multi-dose purposes. BAC water can be sourced from medical supply suppliers in 10 mL and 30 mL vials.
To add BAC water to the peptide vial, pour it carefully through the wall of the vial and make sure not to directly splash on the lyophilized peptide. Swirl lightly for it to dissolve. You can check our injectable peptides guide for detailed instructions.
FAQ, Peptide Calculator
What volume of water should be added?
The majority of protocols opt for either 1 or 2 mL of bacteriostatic water for each vial, with 1 mL yielding a more concentrated solution (smaller volume injections) while 2 mL results in a less concentrated solution (easier for small dose measurement on the insulin syringe). There isn’t a specific volume which can be regarded as correct as it will depend on the required dosage and the level of accuracy necessary. When it comes to very small doses (under 100 mcg), it is best to use 2 mL as it produces better results when using a conventional insulin syringe.
What is the difference between units and milliliters?
Units are a volume measure on insulin syringes: 100 units equals 1 mL on a U-100 syringe. Units are not a measure of peptide content. One unit contains a different amount of peptide depending on the concentration of your reconstituted solution. Always calculate concentration first, then convert your target dose to units.
How accurate are insulin syringes?
Standard insulin syringes (U-100) have markings at every 1 or 2 units. The practical accuracy is approximately ±1 unit, which at 5 mg/mL means ±50 mcg. For research protocols requiring higher precision, 0.3 mL insulin syringes with half-unit markings or tuberculin syringes with 0.01 mL graduations are more accurate. The syringe's accuracy ceiling matters more at lower volumes, a 2-unit draw has higher relative error than a 20-unit draw.
Does the calculator work for any peptide?
Indeed. The reconstitution calculation applies universally. Concentration is defined as milligrams divided by milliliters, no matter the peptide contained in the vial. The calculator is applicable for BPC-157, TB-500, Ipamorelin, Epithalon, MOTS-c, as well as any other lyophilized peptide. There is only one difference while performing the calculation, that is, the amount of peptide indicated in the vial and the amount of water being added in the process.
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